1. Collection and storage of samples Try to use fresh tissue materials. If the collected samples are not used immediately for extraction experiments, please store them at -20°C or -70°C, and avoid repeated freezing and thawing during this period. When using samples that have been repeatedly freeze-thaw or have not been refrigerated for a long […]
Nucleic acid includes two kinds of molecules, DNA and RNA, both of which exist in the state of being bound to proteins. The general principles of nucleic acid separation and purification: 1. The integrity of the primary structure of the nucleic acid should be guaranteed; 2. The contamination of other molecules should be excluded. In […]
1. The purpose of the experiment Clarify the preparation principle of the extract Through the preparation of the extract, grasp the role of each reagent in the extract in the extraction. 2. Experimental principle DNA is genetic material, and the extraction of plant DNA is the basis of plant genetic engineering. It is usually required […]
As the foundation of all molecular biology research, nucleic acid extraction is always the first step in a research. The quality of the extracted nucleic acid is the key to the success or failure of downstream molecular biology experiments. This article will introduce in detail the relevant information about DNA extraction, including the principles of […]
1. Take 100ul-200ul of fresh or frozen muscle tissue or 0.05g of sample tissue preserved in 95% ALC. 2. Cut the tissue as much as possible and put them into 1.5ml centrifuge tubes. 3. Add 450ul of lysis buffer (10mM Tris-HCl pH8.0; 100mM EDTA, pH8.0) to each tube 4. Add 10% SDS 50-100ul (70-80 variable) […]
Paternity Testing DNA typing is the most commonly used for paternity testing. Human blood, hair, saliva, oral cavity cells, etc. can all be used for paternity testing, which is very convenient. A person has 23 pairs (46) of chromosomes. A pair of genes at the same position on the same pair of chromosomes […]
At present, the most effective method for animal molecular research is from the level of nucleic acid molecules, mainly for the identification of species, the origin of species, the assessment of diversity, the study of kinship, system evolution, etc. Due to the limitations of field extraction, many materials must be processed and brought back to […]
The nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is widely used in various fields such as environmental microbial testing, food safety testing, blood transfusion safety, forensic identification, disease control ZX, clinical disease diagnosis, biological research, and animal husbandry. Let […]
DNA extraction is a necessary method in many molecular biology applications. There are many commercial kits that can help with this process. However, through PCR detection, the extraction efficiency of different types of kits is different. We can use machines to assist our experiments-DNA extractor and RNA extractor to ensure better results. Choosing the right kit […]
principle: 1. Precipitate DNA dissolved in NaC1 solution. 2. Use cold alcohol to extract DNA with less impurities. 3. DNA is dyed blue by diphenylamine in the boiling water bath. Method steps: 1. Extract cell nucleus material: stir clockwise, a little faster and a little heavier. 5 min 2. Dissolve DNA: 3. Precipitate a viscous […]
