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DNA extraction:Common problems, causes, solutions

Posted by DNA Extractor on September 25, 2021

The nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is widely used in various fields such as environmental microbial testing, food safety testing, blood transfusion safety, forensic identification, disease control ZX, clinical disease diagnosis, biological research, and animal husbandry. Let the editor take you through the common problems of DNA extraction.


Frequently Asked Questions about DNA Extraction

Low DNA extraction

Reason 1: Incomplete precipitation

Solution: Precipitation at low temperature can prolong the precipitation time. Add auxiliary substances to promote precipitation.

Reason 2: DNA loss during washing

Solution: When washing, use a pipette tip to suck out the cleaning liquid Z, and don't pour it.

Reason 3: The experimental materials are not good or the quantity is small

Solution: Use fresh materials as much as possible.

Reason 4: Broken wall or insufficient cracking

Solution: Animals and plants should be homogenized and grind thoroughly. Before lysis of G+ bacteria and yeast, use enzyme or mechanical methods to break the walls. During high-temperature lysis, prolong the time appropriately.

DNA degradation

Reason 1: The extraction process is violent

Solution: Follow-up operations after cell lysis should be as gentle as possible.

Reason 2: Exogenous nuclease contamination

Solution: Use sterile water to configure all reagents and sterilize consumables at high temperature.

Reason three: repeated freezing and thawing

Store the DNA in aliquots in buffer to avoid repeated freezing and thawing.

Reason 4: The material is not fresh or repeated freezing and thawing

Solution: Use fresh materials as much as possible. In order to prevent repeated freezing and thawing, the materials should be stored at low temperature.

Reason 5: The activity of endogenous nuclease is not very good.

Solution: When extracting DNA from a material rich in endogenous nucleases, the content of chelating agent in the lysate can be increased.


DNA sample is impure

Reason 1: Before the DNA is dissolved, there is alcohol residue, and the alcohol will play a YZ effect on the subsequent enzymatic hydrolysis reaction.

Solution: Re-precipitate DNA and fully evaporate alcohol.

Reason 2: There are metal ions remaining in the DNA

Solution: Increase the number of washing with 70% ethanol by 2-3 times

Reason 3: There are impurities such as protein, polysaccharides and polyphenols in the DNA

Solution: Re-purify the DNA to remove impurities such as protein, polysaccharides and polyphenols.

The fully-automatic nucleic acid system-DNA extractor can solve these problems. The fully-automatic system, without manual intervention, can effectively avoid the above problems.


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