Nucleic acid includes two kinds of molecules, DNA and RNA, both of which exist in the state of being bound to proteins.
The general principles of nucleic acid separation and purification:
1. The integrity of the primary structure of the nucleic acid should be guaranteed; 2. The contamination of other molecules should be excluded.
In order to ensure the study of nucleic acid structure and function, a complete primary structure is the most basic requirement, because genetic information is all stored in the primary structure, and the primary structure of nucleic acid also determines the form of its high-level structure and other biological macromolecules. The way of combining. For the purification of nucleic acid, the following three requirements should be met:
1. There are no organic solvents and excessively high concentrations of metal ions that can inhibit enzymes in nucleic acid samples;
2. The pollution of other biological macromolecules such as proteins, polysaccharides and lipid molecules should be minimized;
3. Eliminate the contamination of other nucleic acid molecules, such as RNA should be removed when extracting DNA molecules, and vice versa.
In order to ensure the integrity and purity of the isolated nucleic acid, the following items should be noted during the experiment:
The main steps of nucleic acid extraction are nothing more than disrupting cells, removing proteins bound to nucleic acids, polysaccharides, lipids and other biological macromolecules, removing other unwanted nucleic acid molecules, precipitating nucleic acids, removing salts, organic solvents and other impurities, and purifying nucleic acids. Wait. The nucleic acid extraction scheme should be determined according to the characteristics of the specific biological materials and the nucleic acid molecules to be extracted. For the nucleic acid molecules enriched in a specific organelle, the scheme of extracting the organelle in advance and then extracting the target nucleic acid molecule can obtain completeness and Nucleic acid molecules with both high purity and high quality.
In the process of DNA extraction, we must abide by the principles. DNA Extractor is a fully automatic nucleic acid extraction system with a variety of models for you to choose from.