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Configuration of dna extraction solution

Posted by DNA Extractor on September 25, 2021

1. The purpose of the experiment

  • Clarify the preparation principle of the extract
  • Through the preparation of the extract, grasp the role of each reagent in the extract in the extraction.

2. Experimental principle

DNA is genetic material, and the extraction of plant DNA is the basis of plant genetic engineering. It is usually required that the extracted DNA has ideal purity, sufficient integrity, no breakage and degradation, and minimal use of toxic chemicals in the extraction process. In order to meet the above requirements, many methods of extracting plant DNA have been reported. In summary, there are mainly CTAB method, SDS method and alkaline extraction method. DNA molecule is the basic material of molecular biology research. Different extraction methods can be used to obtain DNA of varying quantity and quality according to different experimental purposes. Therefore, the ratio of reagents in the extraction solution is also different: the extraction of plant DNA often uses the CTAB method.


The formula is as follows:

1. Before preparing the extract, prepare the mother liquor.

Mother liquor (sterilized): 5M NaCl (58.4g add ddH 2 O to make 200ml solution)

0.5M Na 2 -EDTA (37.22g+ddH 2 O to 200ml, adjust the pH to 8.0 with NaOH)

1M Tris-HCl (12.11g Tris-HCl+ ddH 2 O to 100ml, adjust the pH to 8.0 with concentrated hydrochloric acid)

2. Extraction solution formula


Add: CTAB powder 2g

1M Tris(pH8.0) 10ml

0.5M EDTA(pH8.0) 4ml

5M NaCl 28ml

PVP 1g

β-mercaptoethanol 1ml

Add double distilled water to make the volume to 100ml


3. Extraction solution

Measure phenol, chloroform, and isoamyl alcohol and mix them in a volume ratio of 25:24:1, then measure chloroform and isoamyl alcohol and mix them in a volume ratio of 24:1 to prepare two extracts, each with 100ml

4 0.1xTE

1ml Tris, 20µl EDTA with H 2 O, adjust the pH to 8.0 with HCl, fix the solution, and sterilize

Three, experimental equipment

Beaker, measuring cylinder, volumetric flask, glass rod, straw, pipette, centrifuge tube, refrigerator, autoclave, electronic balance, bottle washing

Four, experimental reagents

Distilled water, CTAB, NaCl, EDTA, Tris-HCl, β-mercaptoethanol, 0.1xTE, RNase enzyme, phenol, chloroform, isoamyl alcohol, absolute ethanol

In summary, it can be seen that manual extraction of DNA is a very complicated and tedious process. If you use DNA extractor, RNA extractor, DNA extraction kit, and RNA extraction kit, you can complete the experiment more efficiently.


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